An extended PCR-RFLP assay for detection of parC, parE and gyrA mutations in fluoroquinolone-resistant Streptococcus pneumoniae
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چکیده
منابع مشابه
An extended PCR-RFLP assay for detection of parC, parE and gyrA mutations in fluoroquinolone-resistant Streptococcus pneumoniae.
Sir, In Gram-positive cocci, fluoroquinolone resistance is associated with mutational alterations in DNA gyrase and/or topoisomerase IV or with active efflux of the drugs. In Streptococcus pneumoniae, fluoroquinolone resistance is mainly due to substitutions in ParC (Ser-79→Phe or Tyr, Asp-83→Asn), ParE (Asp-435→Asn) or GyrA (Ser-81→Phe or Tyr, Glu-85→Lys or Gly).1,2 Therefore, detection of the...
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We have analyzed genetically three clinical isolates (3180, 3870, and 1244) of Streptococcus pneumoniae with high-level ciprofloxacin resistance. Isolates 3180 and 3870 were atypical because of their insolubility in deoxycholate. However, they hybridized specifically with pneumococcal autolysin and pneumolysin gene probes and have typical pneumococcal atpC and atpA gene sequences. Analysis of t...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 2004
ISSN: 1460-2091
DOI: 10.1093/jac/dkh147